Innate Myeloid Cell Immune Response After Prime Boost MVA Vaccination

Goal of this website

This website is complementary to a peer-reviewed scientific publication showing that prime and boost vaccination elicit a distinct innate myeloid cell immune response.

Through the IDMIT data dissemination platform, we give full access to pre-processed and analyzed mass cytometry data to allow readers to re-use, re-explore or analyze deeper one marker or one cell subset of their own interests. An additional goal is to facilitate the interpretation and understanding of our high-dimensional and longitudinal cytometry data and analysis thanks to interactive representations.

In particular, it gives us the opportunity to link more easily cell subsets phenotypes together with their kinetics. Finally, it is a mean to showcase and share the analytical pipeline we have developed specifically to mine longitudinal mass cytometry data.

Overview of the project

A better understanding of the innate immune response to vaccination is critical for the rational design of vaccines. We studied blood innate myeloid cells after first and second immunization, using a preclinical model of non-human primates immunized with a live attenuated vaccine, Modified Vaccinia Ankara (MVA).

We showed that the numbers of blood neutrophils, monocytes, and dendritic cells were transiently impacted by vaccination, as expected, but without any major difference between prime and boost. However, phenotyping deeper those cells with mass cytometry revealed their high phenotypic diversity and their differential response to vaccine injections. It appeared that some cell subsets were enriched only after the primary injection and others only after the boost, and some both after prime and boost. Actually, while circulating innate myeloid cells rapidly returned to baseline level in terms of their number after prime, their sub-phenotype composition was modified over time, and finally differed at the time of the boost. We next analyzed the markers co-expression profile of cell subpopulations best characterizing the ‘primary’ and ‘secondary’ innate myeloid responses. We showed that innate myeloid cells responding to the second immunization were more activated and mature.

Our study revisits innate immunity by demonstrating that, like adaptive immunity, innate myeloid responses differ after one or two immunizations.