Data dissemination

Originality and quality of the dataset

Our mass cytometry dataset is composed of macaque blood samples collected at steady state, before or long after immunization, outnumbered by samples collected very early after immunization, during acute inflammation.

To ensure a high quality of the dataset, we controlled the quality of each staining and acquisition and their reproducibility. We used the same batches of antibody all along the longitudinal study and we included the same two control samples each time to allow us to exclude samples or markers from the analysis because of a batch effect.

Data accessibility

We provide full access to our mass cytometry data here (pre-processed and processed data).

Data are also available from Flowrepository (ID FR-FCM-ZYBG) , which also provides full experimental and acquisition metadata.

Data are also accessible from Cytobank under accession number 68443 and 68590. Cytobank Community allows new analyses using manual gating. We created gates for each phenotypic family and superfamily, so you can visualize our data (phenotype and cell number) at these two levels and decide which marker, animal, and time-points you want to display as histograms, including overlays, or biplots. Cytobank Premium and Enterprise allow additional multidimensional analyses using SPADE, but also viSNE and CITRUS, although without a random pre-downsampling step, which we believe is key to equalize the contribution of each sample and which was included in the current analysis.