NK Cell Response After Prime Boost MVA Vaccination

Goal of this website

This website is complementary to a peer-reviewed scientific publication showing that prime and boost vaccination elicit a distinct NK cell immune response.

Through the IDMIT data dissemination platform, we give full access to pre-processed and analyzed mass cytometry data to allow readers to re-use, re-explore or analyze deeper one marker or one cell subset of their own interests. An additional goal is to facilitate the interpretation and understanding of our high-dimensional and longitudinal cytometry data and analysis thanks to interactive representations.

In particular, it gives us the opportunity to link more easily cell subsets phenotypes together with their kinetics. Finally, it is a mean to showcase and share the analytical pipeline we have developed specifically to mine longitudinal mass cytometry data.

Overview of the project

We studied blood NK cells after first and second immunization, using a preclinical model of non-human primates immunized with a live attenuated vaccine, Modified Vaccinia Ankara (MVA).

NK cell number decreased after each immunization, but without any major difference between prime and boost. However, in depth phenotyping of NK cells with mass cytometry revealed a high diversity in their phenotypes. Some NK cell subphenotypes responded to only one of the two immunizations. Actually phenotypes responding to the boot differ from those responding to the prime.

Consistently with our data on innate myeloid cells, we showed that NK cell response differ at each immunization with subphenotypes responding to the boost being more activated than those responding to the prime.