After the two successive clusterings and the identification of the phenotypic families discriminating between the prime and boost responses, the last step of our longitudinal mass cytometry data analysis was to compare the marker expression between the two classes.

We identified and ranked the markers differentially expressed between ‘primary‘ and ‘secondary‘ NK cells, based on the Kolmogorov-Smirnov distance.

As cytometrists and immunologists, we ultimately want to see histograms! The expression patterns of top markers can be displayed in the different phenotypic families.

Note that each histogram include samples for the entire dataset, please look at the heatmap and the corresponding kinetic patterns to see more about the dnamivs of those subphenotypes.

For dot-plots and overlay representations, clusters, samples or other marker histograms, visit our shared Cytobank experiments, since we opted to focus here solely on discriminant markers in the context of our study.